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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in lots of tumors, which results in chromosome instability, and thus could lead to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a significant purpose in promoting mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have been explained. Two extra splice variants have been claimed but specific nucleotide sequence details is not really nonetheless readily available.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically incorporates the epitope identified through the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This tutorial outlines further feasible optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies bound to the blocking peptide no longer bind for the epitope within the goal protein. By evaluating the staining from your blocked antibody versus the antibody alone, you can see which staining is distinct.
Concentrate on Info Cyclin E belongs on the highly conserved cyclin family members, whose members are characterized by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins show unique expression and degradation designs which lead on the temporal coordination of every mitotic function. Cyclin E types a complex with and features as being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates for the G1-S section boundary which is degraded as cells progress by S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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